摘要:competent cells (DH5alpha at competency of 108 cfu/µl of pUC18). The transformation procedure it self is very similar to a standard CaCl2 transformation. Place 100 µl of TB buffer in a tub[阅读全文]

摘要:The overall sequence of events is:• Titer and plate out phage • Lift plaques onto filters and prepare them for screening • Make a probe • Hybridize the probe to the filters • [阅读全文]

摘要:Caution: EtBr is a powerful mutagen and is moderately toxic. Wear gloves when working. When EtBr conc. is >0.5 mg/ml: 1.Add sufficient water to reduce the concentration of EtBr to <0.5 mg/ml. 2.Add 1 [阅读全文]

摘要:Hello group!I want to insert a small 30 bp sequence into a vector of mine using ds-oligo. I've created the oligos so that once annealed, they have the correct overhangs of the restriction sites.As I'm[阅读全文]

摘要:Hey folks,I need Help!! Please!I have to put a 3.5 kB fragment into a 6.5 kB vector.The vector has a Km resistance gene.I use invitrogen chemically competent cells.This is what I do:I PCR amplify the [阅读全文]

摘要:Steven Finkbeiner,Departments of Neurology and Physiology,UCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/cloning.shtml DIGEST:1.Digest 10 - 20μg DNA to isolate either the desired[阅读全文]

摘要:1.Wash the plates briefly using the 96-channel block washer. 2.Place the plates in a tub submerged in dd-water. 3.Put a flask on top of the plates to keep them submerged. 4.Place the tub with the subm[阅读全文]

摘要:hi there,I have been trying to clone 3.4 kb insert into pcDNA3.1- for months without any success.The insert is subcloned in TOPO from where I cut it out by KpnI and NotI and purify it by gelelectropho[阅读全文]

摘要:本课件介绍了克隆载体类型、特点及载体必不可少的基本元件。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]