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搜索“TAG:cloning”找到相关内容6篇,用时0.011075秒
摘要:competent cells (DH5alpha at competency of 108 cfu/µl of pUC18). The transformation procedure it self is very similar to a standard CaCl2 transformation. Place 100 µl of TB buffer in a tub[阅读全文]
摘要:Hello group!I want to insert a small 30 bp sequence into a vector of mine using ds-oligo. I've created the oligos so that once annealed, they have the correct overhangs of the restriction sites.As I'm[阅读全文]
摘要:Hey folks,I need Help!! Please!I have to put a 3.5 kB fragment into a 6.5 kB vector.The vector has a Km resistance gene.I use invitrogen chemically competent cells.This is what I do:I PCR amplify the [阅读全文]
摘要:Steven Finkbeiner,Departments of Neurology and Physiology,UCSF http://gweb1.ucsf.edu/labs/finkbeiner/Protocol/protocol_list/cloning.shtml DIGEST:1.Digest 10 - 20μg DNA to isolate either the desired[阅读全文]
摘要:hi there,I have been trying to clone 3.4 kb insert into pcDNA3.1- for months without any success.The insert is subcloned in TOPO from where I cut it out by KpnI and NotI and purify it by gelelectropho[阅读全文]
摘要:本课件介绍了克隆载体类型、特点及载体必不可少的基本元件。本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]