摘要:DNA PrepPrepare DNA via your favorite method.You may find a protocol underMini Yeast Genomic Prep.Restriction Digest1.Digest DNA with appropriate restriction enzyme.Digest 10μg of Yeastchromosomal [阅读全文]

摘要:1. Run the gel as normal. Often for genomic southerns it is desirable to run long gels (18cm) over 4-6hrs.2. Photograph the gel with a ruler adjacent to the molecular weight markers as a reference.3. [阅读全文]

摘要:Southern Analysis Procedure (for analysis of genomic DNA or ordering of clones): 1) Prepare genomic DNA (ref.p.9.22, Maniatis) from cultured cells using one T75 flask (wash two times with PBS and then[阅读全文]

摘要:核酸分子杂交技术用一段核酸片段带上特殊标记物后，在一定温度，离子强度，PH条件下，与相同or相似的核酸分子以硷基互补原则（C≡G, A＝T,A＝U）形成新的杂合分子的过程，以检测核酸分子的存在。根据反应性质分为：RNA-RNA, RNA-[阅读全文]

摘要:在胶体中的DNA 可利用毛细现象的作用将DNA 牵引转印至覆盖在胶片上的滤膜，经烘烤或UV 照射后，可使DNA 固定在膜上，以进行探针的杂合（hybridization） 反应。仪器用具：玻璃缸；玻璃板或吸水海绵；Crosslinker （Stratalinke[阅读全文]

摘要:PCR screens must be designed to detect transgene DNA at the single copy level. Southern Blots analysis of transgenic mice need copy standards to estimate copy number. Copy standards are prepared by mi[阅读全文]

摘要: DNA的提取与检测人和哺乳动物细胞基因组DNA的分离通常是在有EDTA、Sarkosye等一类去污剂存在下，用蛋白酶K消化细胞，随后用酚、氯仿抽提，经RNase处理和纯化来提[阅读全文]