Composition per liter:

NaCl ……19.45g

Agar ……15.0g

MgCl₂……8.8g

Peptone……5.0g

Na₂SO₃……3.24g

CaCl₂……1.8g

Yeast extract ……1.0g

KCl……0.55g

NaHCO₃ ……0.16g

Ferric citrate……0.1g

KBr……0.08g

SrCl₂……0.03g

H₃BO₃ ……0.02g

Na₂HPO₄ ……8.0mg

Na₂SiO₃……4.0mg

NaF……2.4mg

NH₄NO₃ ……1.6mg

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of Hyphomonas spp., Oceanospirillum spp., Hyphomicrobium indicum, Psychroflexus gondwanensis=Flavobacterium gondwanense, Salegentibacter salegens=Flavobacterium salegens, Psychromonas antarctica, Sulfitobacter mediterraneus, Thalassomonas viridans,Vibrio spp., Marinospirillum minutulum=Oceanospirillum minutulum, Terasakiella pusilla=Oceanospirillum pusillum, Pseudoalteromonas atlantica=Alteromonas atlantica, Pseudomonas atlantica,Roseobacter spp., Erythrobacter longus, Pseudospirillum japonicum=Oceanospirillum japonicum,Marinobacter hydrocarbonoclasticus （Pseudomonas nautica）， Psychrobacter spp., and Moritella japonica.

For the isolation, cultivation, and maintenance of a wide variety of heterotrophic marine bacteria.