TGEK Base 50 mM Tris 10% vol Glycerol 1 mM EDTA 100 mM KCl (add for std lysis buffer): PMSF Benzamidine Leupeptin Aprotinin 0.5% NP40 (add for solubilization buffer) 1% NP40 0.1% Triton X100 0.1% SDS 0.5% NaDeoxycholate Buffer A (fractionation buffer) 10 mM Tris 1.5 mM MgCl2 10 mM KCl Buffer C (nuclear lysis buffer) 25% glycerol 1.5 mM MgCl2 0.2 M EDTA 0.42 M NaCl 10 mM Tris RIPA 50mM Tris HCl 150 mM NaCl 1% NP40 0.5% NaDeoxycholate 0.1% SDS Ubi Buffer 1 (base) 50 mM Tris pH 7.4 1 mM EDTA 250 mM NaCl 0.1% NP40 1% Triton X100 0.5% SDS 0.25% DOC (add) 100 mM NaF 5 mM NaVO3 Pepstatin PMSF Aprotinin Benzamidine Leupeptin Ubi Buffer 2 (base) 50 mM Tris pH 7.4 1 mM EDTA 0.1% NP40 250 mM NaCl (add) 5 mM NaF 0.5 mM NaVO3 Pepstatin PMSF Aprotinin Benzamidine Leupeptin NP40 20 mM Tris pH 7.5 10 mM B-Glycerophosphate 2 mM EGTA 5 mM NaF 150 mM NaCl 1 mM NaPPi 1% NP40 (add) 2 mM DTT 1 mM Vanadate .5% Aprotinin 100 uM PMSF (or for IP,add) Aprotinin Pepstatin Benzamidine Leupeptin PMSF 5mM Vanadate 5mM DTT |
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