Gregory Hannon, Cold spring harbor lab

Our overall approach is to use an RNA polymerase III promoter to drive

expression of encoded short hairpin RNA (shRNA). For this purpose we use the human

U6 snRNA promoter, maintaining the transcript initiating “G” nucleotide of the

U6snRNA transcript. There by, hairpin sequences will start with a “G”. Termination is

mediated by a run of Ts at the end of the hairpin.

Web_version_of_PCR_strategy1.pdf