生命经纬知识库 >>所属分类 >> DNA技术   

Ethanol Precipitation of DNA 乙醇沉淀DNA【University of Texas 】

标签: 暂无标签

顶[0] 发表评论(45) 编辑词条

Kitto Lab, The University of Texas at Austin http://research.cm.utexas.edu/bkitto/Kittolabpage/Protocols/Microbiology/ethanolPpt.html

This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.

Materials

•3M Sodium Acetate buffer, pH 5.2 (store at 4 ℃)

•Cold 100% Ethanol (-20℃)

•Cold 70% Ethanol in sterile dH2O (-20℃)

•DNA sample

•4 ℃ Microcentrifuge (normal microcentrifuge in cold room works fine). All centrifugations should be on "soft" (no brake) setting.

Procedure

1.Transfer DNA to a container where it fills one fourth the total volume (a 500 μl tube should have no more than 125 μl of DNA solution, for example).

2.Add one tenth volume of Sodium Acetate buffer to equalize ion concentrations.

3.Add at least two volumes of cold 100% ethanol; let stand in -20℃ freezer for at least one hour .

4.Centrifuge sample for 15 minutes at highest speed in a 4℃ microcentrifuge .

5.Remove as much supernatant as possible with a 1 mL micropipet; recentrifuge, then remove the rest with a 200 μl pipet.

6.Add 200 μl of cold 70% ethanol; centrifuge for 5 minutes in a 4 ℃ centrifuge.

7.Remove supernatant with a 200 μl pipet; evaporate remaining ethanol in a 37 ℃ water bath.

8.Resuspend pellet in desired volume of water or TE buffer.

附件列表


→如果您认为本词条还有待完善,请 编辑词条

上一篇DNA实验室的故事之一:远古DNA 下一篇电击转感受态的制备

词条内容仅供参考,如果您需要解决具体问题
(尤其在法律、医学等领域),建议您咨询相关领域专业人士。
0

收藏到:  

词条信息

admin
admin
超级管理员
词条创建者 发短消息   

相关词条