标签: Purification
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1.Remove gel slice contain DNA fragment and place in 10 volumes of: 300 mM NaOAc,pH 7.0 300 ml 1 M NaOAC,pH 7.0 1 mM EDTA 2 ml 500 mM EDTA,pH 8.0 698 ml ddH20 2.Incubate at 22℃ for 30 min.Transfer gel slice to a fresh tube. 3.Place tube in a Dry Ice/Ethanol bath for 5 min. 4.Puncture the bottom of a 0.5 ml microcentrifuge tube with a needle.Place the gel slice into this tube.Place this tube inside a 1.5 ml microcentrifuge tube. 5.Centrifuge for 15 min. 6.Collect the Eluent from the 1.5 ml eppendorf tube.Extract and precipitate the DNA. |
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上一篇Plasmid Mini Purification Protocol(质粒纯化) 下一篇Oligo?-?Storage?and?Handling
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