标签: Silinization 硅化玻璃
顶[0] 发表评论(16) 编辑词条
Cepko/Tabin Lab ,Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/S6.html Superfrost plus slides and standard slides can be silinized but I have found that superfrost slides work best so that protocol is outlined here. Solutions Reagents Needed Superfrost Plus Slides TESPA-3 aminopropyltrioxysilane (Sigma # A3648) acetone EtOH Concentrated HCl Procedure • Dip a rack of superfrost plus slides in 2N HCl (200 ml Q 40 ml concentrated HCl)for 1 minute. • Rinse the slides for 1 minute in Milli Q water. • Rinse the slides in 100% Acetone for 1 minute. • Incubate in 100% Acetone containing TESPA for 1 minute. • Rinse briefly in 100% acetone. • Rinse briefly in 95% EtOH followed by 50% EtOH,30% EtOH and several changes of Q. • Air dry at room temperature overnight and then store at 4℃ dessicated until use.They keep for several months. |
→如果您认为本词条还有待完善,请 编辑词条
上一篇Genomic?DNA?Quickprep?for?PCR 下一篇Restriction?Digests?of?Genomic?DNA
词条内容仅供参考,如果您需要解决具体问题
(尤其在法律、医学等领域),建议您咨询相关领域专业人士。
0