Quick Isolation of DNA from Agarose Gels

Hancock Laboratory Methods. Department of Microbiology and Immunology,

University of British Columbia, British Columbia, Canada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=67

METHOD:

1.Run gel as normal in 1X TBE.

2.Visualize band under LONG wave UV.

3.Cut band out with razor blade.

4.Place excised agarose slice in an Eppendorf tube, which has a hole in its bottom (by inserting a hot needle), and which has a small amount of siliconized glass wool covering the opening.

5.Place this tube inside another Eppendorf tube.

6.Spin tubes either at 1/2 maximum speed for 15 minutes or, if not possible, full speed for 5 minutes.

7.Phenol/chloroform the resulting liquid.

8.Repeat with 1/10 volume 3 M NaAc 2x volume EtOH.