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Cell Lysates For Western Blotting
词条创建者:admin创建时间:12-09 22:10
标签: Cell Lysates Western Blotting

摘要:Reagents / Solutions Lysis Buffer:10ml 10% Sodium dodecyl sulphate (SDS)10ml Glycerol10ml b-mercaptoethanol8ml 0.5M Tris pH6.81ml 0.1% bromophenol blue51ml H2OProtocolSpin down 106 cells and wash, if [阅读全文]

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CELLDYN 1400型血球计数器故障检修
词条创建者:admin创建时间:12-08 23:24
标签: 血球计数器 维修

摘要:1、故障现象开机后自检一切正常,测试病人标本时,白细胞检测不能通过,wbc栏显示:Flow Err(计数时间过短)。2、分析与检修经清洗管路、更换新试剂后,重新开机测量标本,故障依旧。将定量管的位置向下移动少许(人为延长T1时间)[阅读全文]

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Cellular ELISA Protocol
词条创建者:admin创建时间:12-08 01:15
标签: Cellular ELISA Protocol

摘要:A. Formalin Fixed Cell Plates1.Trypsinize confluent flasks2.Pool and count cells3.Centrifuge at 1500 rpm for 10 minutes4.Resuspend to the appropriate concentration in complete medium4 x 105 cells/ml f[阅读全文]

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Cell Cultures
词条创建者:admin创建时间:12-08 00:48
标签: Cell Cultures

摘要:IntroductionA major advance in our knowledge of cells came about with the ability to maintain them in continous culture. Prokaryotes have been cultured for a relatively long time, but eukaryotic cultu[阅读全文]

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Cell counting with an hemacytometer.
词条创建者:admin创建时间:12-08 00:47
标签: 血细胞计数器 细胞计数 cell counting hemacytometer.

摘要:Accuracy of manual counts with an hemacytometer depend on:accurate mixing of the sample (no bubbles!)number of chambers countednumber of cells counted (practical: 200-500 per 0.1 mm3)The chamber of va[阅读全文]

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CELLULAR ELECTRON MICROSCOPY
词条创建者:admin创建时间:12-08 00:44
标签: CELLULAR ELECTRON MICROSCOPY

摘要:Description Recent advances in the imaging technique electron microscopy (EM)have improved the method,making it more reliable and rewarding,particularly in its description of three-dimensional detail。[阅读全文]

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Cell Culture Protocols
词条创建者:admin创建时间:12-08 00:43
标签: Cell Culture Protocols

摘要:1.Basic Techniques - The “Do’s and Don'ts” of Cell CultureGiven below are a few of the essential "do’s and don’ts" of cell culture. Some of these are mandatory e.g. use o[阅读全文]

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Cell culture及Cell Culture Troubleshooting(电子版)
词条创建者:admin创建时间:12-08 00:40
标签: cell culture

摘要:马歇尔大学的细胞培养培训幻灯,有理论,有实践,是细胞培养上手的一份好教材。特地推荐给准备培养细胞的新人,大家共同学习。点击下载:Growing PLHC1 Cells in Culture.ppt还有“Cell Culture Troubleshooting&rdquo[阅读全文]

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