按字母顺序浏览:A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 其他

搜索“TAG:LABELING”找到相关内容11篇,用时1.057836秒

词条| 搜标签
Labeling oligonucleotides with 32P ATP
词条创建者:admin创建时间:12-09 23:24
标签:

摘要:Wear gloves throughout and work in radiation area. Monitor area before and after use.Mix the following in an eppendorf tube:1. 0.5 microgram oligonucleotide dissolved in H2O.2. 3 microliters 10x kinas[阅读全文]

编辑:0次| 浏览:1269次
Labeling?oligonucleotides?with?32P?ATP
词条创建者:admin创建时间:12-09 23:21
标签: Labeling oligonucleotides 32P ATP

摘要:Wear gloves throughout and work in radiation area.Monitor area before and after use.Mix the following in an eppendorf tube:1、0.5 microgram oligonucleotide dissolved in H2O.2、3 microliters 10x kinase b[阅读全文]

编辑:0次| 浏览:855次
LABELING GC-GLOBULIN WITH FLUORESCEIN
词条创建者:admin创建时间:12-09 22:08
标签: GC-GLOBULIN FLUORESCEIN

摘要:Materials1.2 mM PIPES,pH 7.0,500 ml.2.Gc-globulin (Calbiochem 345802),1 mg.3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes).4.Lysine,100 mM in buffer 1,10 ml.5.G25-150 desalting column[阅读全文]

编辑:0次| 浏览:716次
Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
词条创建者:admin创建时间:12-09 22:08
标签: Tetramethylrhodamine Succinimidyl Ester

摘要:Materials1.Drop frozen 2x cycled tubulin in a polymerization-promoting buffer.Stored at -80℃2.GTP,100 mM stock,titrate to pH ~7.Need 2 ml.3.Waterbath,set at 37℃4.SS34 rotor,5 ml and 15 ml tubes and ad[阅读全文]

编辑:0次| 浏览:988次
LABELING ALPHA-ACTININ WITH IATR
词条创建者:admin创建时间:12-09 22:08
标签: ALPHA-ACTININ IATR

摘要:Materials1.Small vial and stir bar.2.Bio-Beads SM-2 in 0.7x15 cm column.3.2 mM Tris-HCl,pH 8.5,4℃,250 ml.4.Centricon-30 concentrator.5.5 mM Tris-acetate,0.1 mM DTT,pH 6.95,4℃,250 ml.6.200 mM K-borate,[阅读全文]

编辑:0次| 浏览:903次
Labeling Vinculin with TRITC
词条创建者:admin创建时间:12-09 22:08
标签: Vinculin TRITC

摘要:Materials1.200 mM K-borate,pH 9.0,10 ml.2.100 mM lysine,100 mM K-borate,pH 9.0,1 ml aliquots stored at -20℃.3.2 mM Tris-HCl,pH 8.5,4℃,250 ml.4.100 mM DTT stock.5.Centricon-30 concentrator.6.5 mM Tris-[阅读全文]

编辑:0次| 浏览:813次
LABELING GIZZARD MYOSIN WITH IATR (MHC AND 17KD MLC)
词条创建者:admin创建时间:12-09 22:08
标签: GIZZARD MYOSIN IATR

摘要:Day 1-2Materials1.0.5 M KCl,10 mM HEPES,pH 7.5,4℃,250 ml.2.0.5 M KCl,50 mM HEPES,pH 8.0,4℃,250 ml.3.Bio-Beads SM-2 in a 0.7x15 column.4.20 mM KCl,20 mM PIPES,pH 7.0,4℃,250 ml.5.IATR (tetramethylrhodam[阅读全文]

编辑:0次| 浏览:788次
Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
词条创建者:admin创建时间:12-09 22:08
标签: Muscle N-(1-pyrene)iodoacetamide

摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0.2.0.5 mM ATP,2 mM MgCl2,100 mM boric acid,pH 8.3 at 4℃,10 ml.3.N-(1-pyrene)iodoacetamide (Molecular probes P-2[阅读全文]

编辑:0次| 浏览:922次
LABELING MUSCLE ACTIN WITH IATR
词条创建者:admin创建时间:12-09 22:08
标签: LABELING MUSCLE IATR

摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0 and 1000 ml for day 1.2.100 mM KCl,100 mM boric acid,pH 8.4 at 4℃,10 ml.3.IATR (tetramethylrhodamine iodoaceta[阅读全文]

编辑:0次| 浏览:706次
Labeling Muscle Actin with 5-iodoacetamidofluorescein
词条创建者:admin创建时间:12-09 22:08
标签: Labeling Muscle 5-iodoacetamidofluorescein

摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0.2.100 mM KCl,100 mM boric acid,pH 8.4 at 4℃,10 ml.3.0.5 mM ATP,2 mM MgCl2,100 mM KCl,2 mM DTT,2 mM PIPES,pH 7.[阅读全文]

编辑:0次| 浏览:734次
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
词条创建者:admin创建时间:12-09 22:08
标签: LABELING MUSCLE CARBOXYFLUORESCEIN SUCCINIMIDYL

摘要:Day 0 and 1Materials1.0.5 mM ATP,0.2 mM CaCl2,1 mM PIPES,pH 6.8 at 4℃,250 ml for day 0.2.0.5 mM ATP,0.2 mM CaCl2,70 mM KCl,150 mM PIPES,pH 7.0,1 ml.3.16 mM ATP,0.2 mM CaCl2,5 mM DTT,5 mM lysine,10 mM [阅读全文]

编辑:0次| 浏览:887次

更多详情,尽在互动百科
更多详情,尽在谷歌搜索