摘要:Protocol for Annealing OligonucleotidesOligo Name:Lot Number:Total nmol:Volume of Annealing Buffer added:Oligo Name:Lot Number:Total nmol:Volume of Annealing Buffer added:Annealing Buffer: 10mM Tris, [阅读全文]

摘要:Oligo designThe 5' end (the homology arm) - choose 42 or more (we usually choose about 50) nts for the homology arms from the target DNA sequence simply according to where you want to insert the PCR f[阅读全文]

摘要:1、Oligo design（1）The 5' end (the homology arm) - choose 42 or more (we usually choose about 50) nts for the homology arms from the target DNA sequence simply according to where you want to insert the [阅读全文]

摘要:Protocol for Isolation of Genomic DNA from Mammalian Cells1.Trypsinize, harvest and resuspend cells at 107 / ml in 10 mM Tris-HCl (pH 8.0), 10 mM EDTA. 2.Add SDS and Proteinase K to a final concentrat[阅读全文]

摘要: Protocol for the cloning of pSHAG-MAGIC2 shRNAsGregory Hannon,Cold spring harbor labpSHAG-MAGIC2.doc [阅读全文]

摘要:Protocol for Sequencing Very Difficult Regions Ziyun Yao and B.A. Roe 02-14-2002Target DNA (SAP-ExoI cleaned PCR 7-deaza-dG containing product)4μl Primer (from mermade) (200 pmoles of primer)1μl[阅读全文]

摘要:PROTOCOL TO EXTRACT DNA FROM PARAFFIN BLOCKS1.Cut 10-20X10μm sections of formalin fixed paraffin samples into eppendorf tubes.2.Add 1 ml xylene, mix, incubate at 55℃ for 15mins. Release pressure, s[阅读全文]

摘要:Prepare in a sterile tube: template RNA:total RNA0.1-5µgor poly(A)+ mRNA10ng-0.5µg,or specific RNA0.01pg-0.5µg primer:oligo(dT)180.5µgor random hexamer 0.2µg,or sequence-[阅读全文]

摘要:Preparation of CellsPrepare or collect between 2x107 cells for each mRNA prep （will yield about 10-20μg of mRNA）. If PBMCs from a whole blood sample are to be used, the sample should be prepared wi[阅读全文]

摘要:1. Prepare a solution of 10X annealing buffer.10 mM MgCl2 , 200 mM Tris-HCl, pH 8.0 2. Prepare an aliquot of the oligonucleotide probe in an eppendorf tube.3. Add a molar excess of oligonucleotide tar[阅读全文]

摘要:This protocol describes the detailed experimental procedure for real-time RT-PCR using SYBR Green I as mentioned in Xiaowei Wang and Brian Seed (2003) A PCR primer bank for quantitative gene expressio[阅读全文]

摘要:Healthy Sp2/0 cells should be rapidly growing by this time. Sp 2/0 cells should be started about two weeks before the cell fusion. Every two days, they should be centrifuged at a 64.4 xg on the IEC cl[阅读全文]

摘要:The Solexa sequencing platform comprises all hardware, software, procedures and kitted reagents for DNA sequencing at the whole genome or repetitive regional scale.a) Preparation of full diversity lib[阅读全文]

摘要:1. Dissect embryos and place each yolk sac in a microfuge tube (can be frozen at -20°C prior to extraction). Original method cited for mouse toes, or 2 mm tail, Reagents: A) 25 mM NaOH / 0.2 mM ED[阅读全文]