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摘要:Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the fo[阅读全文]

摘要:Materials:10X restriction enzyme buffer (see manufacturer's recommendation)DNAsterile waterrestriction enzymephenol:chloroform (1:1) 1.Add the following to a microfuge tube:2 μl of appropriate 10X [阅读全文]

摘要:Restriction enzyme digestions are performed by incubating double-stranded DNA molecules with an appropriate amount of restriction enzyme, in its respective buffer as recommended by the supplier, and a[阅读全文]

摘要:Restriction enzymes are expensive ($40 to $200 a vial): keep the enzymes at -20℃. Plan your digests to be small and convenient. The digest is composed of DNA sample (volume should not be more than abo[阅读全文]

摘要:The Preuss Lab,The Division of Biological Sciences,The University of Chicago.http://preuss.bsd.uchicago.edu/protocols/enzyme.html[阅读全文]

摘要:(adapted from Bruce A. Roe, Department of Chemistry and Biochemistry, The University of Oklahoma, Norman, Oklahoma 73019 broe@ou.edu)Restriction enzyme digestions are performed by incubating double-st[阅读全文]

摘要:The Minion Lab, College of Veterinary Medicine at Iowa State Universityhttp://mycoplasmas.vm.iastate.edu/lab_site/methods/DNA /restrdig.html [阅读全文]