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摘要:We use two different kinds of media. Most cells are grown in DMEM. A few lymphoid cell lines are grown in RPMI. Cells grown in DMEM must be grown in a 10% CO2 atmosphere. As a result, most of our incu[阅读全文]
摘要:MS MEDIUM FOR ARABIDOPSISTo 990 ml H2O add:Sucrose ........... 10.0gMOPS..............0.5gAgar..............8.0gAdjust pH to 5.7 with 1 M KOH.Autoclave.When cooled add sterile stock solutions of:KNO3.[阅读全文]
摘要:METHOD:1.Established hybridomas and other cell lines can be stored in liquid N2 using this method.2.Have everything ready before you start working with the cells (i.e. cool media, label vials, prepare[阅读全文]