摘要:Acid Washing Beads Peter Novick Lab, Department of Cell Biology Yale University School of Medicine http://info.med.yale.edu/cellbio/Novick/Second/Protocols/AcidBeads.pdf Materials:1) 0.5 mm glass bead[阅读全文]

摘要:This is the preferred method for yeast RNA preparation use Gloves and RNAse free solutions throughout.1. Use a YPD overnight culture to innoculate fresh YPD media and grow cells at 30 degrees overnigh[阅读全文]

摘要:Acid Adjusted Swine Serum1. The before or morning of: adjust the pH of the swine serum to between 4.3 and 4.5 with 1N HCl.2. Place serum for at least 1 hour at 4°C (overnight is best).3. Centrifug[阅读全文]