摘要:1. set up the following reaction: CIP RxnH2 O 7.8 ml 10x cip rxn buffer 2.0 ml DNA(e.g; 3 kb vector; 0.2 mg/ml; 2 mg total) 10.0 ml (1 u/ml) CIP 0.2 ml total 20.0 ml [阅读全文]