摘要:Preparation of Transformation Competent DNA Dilute an overnight bacterial culture 1:20 in L broth. (For E. coli strain JA221, at A )Using a serological pipet, resuspend the bacterial pellet gently in [阅读全文]

摘要:1. Primers:i) Oligonucleotide primers are generally supplied as "so many OD units/ml" - but what does this mean, in terms of mg/ml, or mmol/ml, etc?Given: a primer is Y nucleotides (nt) long;Given: th[阅读全文]

摘要:《Caliper-EZ Reader在药物筛选领域的应用》下载点击这里下载[阅读全文]

摘要:Steven Finkbeiner, Departments of Neurology and Physiology, UCSF Protocol note: hood scgedule for day of transfection: [2h] - 6h gap - [1h] CELLS: Polylysine coat plates. add 5ml polylysine per 100mm [阅读全文]