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摘要:Preparation of Transformation Competent DNA Dilute an overnight bacterial culture 1:20 in L broth. (For E. coli strain JA221, at A )Using a serological pipet, resuspend the bacterial pellet gently in [阅读全文]
摘要:Steven Finkbeiner, Departments of Neurology and Physiology, UCSF Protocol note: hood scgedule for day of transfection: [2h] - 6h gap - [1h] CELLS: Polylysine coat plates. add 5ml polylysine per 100mm [阅读全文]