按字母顺序浏览:A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0 1 2 3 4 5 6 7 8 9 其他
搜索“TAG:ELECTROPORATING”找到相关内容1篇，用时0.010492秒
摘要:1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃2.Grow 2-3H to A660 ~0.3-0.43.Chill cells 10'/ice Spin down 10',5K,4℃4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃5.Wash 1X 40 ml 10% gl[阅读全文]