摘要:Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate thi[阅读全文]

摘要:Protocol for extracting DNA from ES Cells,starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA.NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this w[阅读全文]

摘要:The Queensland Institute for Medical Research (QIMR) lab's method for automated sequencing for expressed sequence tags from Schistosoma japonicum and/or S. mansoni cDNA libraries constructed in the di[阅读全文]

摘要:EST：Expressed Sequence Tag，即序列表达的标签。[阅读全文]

摘要:MaterialsChick embryo (approximately 8 days old)70% (v/v) ethanol for swabbingSterile scissors, forceps and probesSterile petri platesPhosphate buffered saline (PBS)Trypsin, cold sterilized in a 125 m[阅读全文]

摘要:Day 0One frozen vial of Murine Embryonic Fibroblasts (MEFs) is thawed quickly in a 37oC water bath. When the last bit of ice is melted, spray the vial with 70% ethanol and transfer the contents of the[阅读全文]

摘要:The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor[阅读全文]