摘要:Mutagenesis is a fundamentally important DNA technology which seeks to change the base sequence of DNA and test its effect on gene or DNA function. The mutagenesis can be conducted in vivo (in studies[阅读全文]

摘要:I am working with very small amount human tissue. Is there anyone who had the experience of using glycogen with 100% ethanol in pheno/chloroform DNA extraction protocol ? Does it realy work to increas[阅读全文]

摘要:In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosineCross-references are to Schwarzacher & Heslop-Harrison 2000. Practical in situ Hybridization. Bios, Oxford[阅读全文]

摘要:实验步骤： 1、Inoculate from an overnight grown in LB.从培养过夜的LB平板上挑取单菌落 。2、Grow in 250 ml "SOB" at 18℃ until OD600 = 0.6.(0.3)接种于250ml SOB，18度培养至OD＝0.6。3、On ice for 10 minutes.菌液置[阅读全文]

摘要:Steve HahnLast Modified Fri,Apr 25,2003Wear gloves throughout,use RNAse free solutions (either autoclaved or sterile filtered)and clean bench and pipetmen with 95% ethanol before use to eliminate any [阅读全文]

摘要:Ⅰ.Isolation of Single Stranded DNA1.Transform plasmid to be mutagenised into JM109. JM109 is endA1, recA1, gyrA96, thi, hsdR17(rk-, mk+), relA1, supE44, l-, d(lac-proAB), [F', traD36, proA+B+, lacIqdM[阅读全文]

摘要:应用已知碱基序列并带有标记物的核酸探针与组织、细胞中待检测的核酸按碱基配对的原则进行特异性结合而形成杂交体，然后再应用与标记物相应的检测系统，通过组织化学或免疫组织化学方法在被检测的核酸原位形成带颜色的[阅读全文]

摘要:In vitro transcription with yeast nuclear extract Steve HahnLast Modified Fri, Apr 25, 2003Wear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and [阅读全文]

摘要:in vitro TranscriptionReaction1 µl 10X Transcription Buffer (Ambion) 1 µl 10X NTPs (4 mM ATP, CTP, 1 mM GTP, UTP) 2 µl 10 mM GpppG cap (Pharmacia) 2 µl a[32P]-UTP (NEN) 800 Ci/[阅读全文]

摘要:The following protocol is for MEGAscript II Kit (Ambion). 1. PCR amplify the DNA template. The 5'-end of the template should contain the minimum promoter sequenences of T7 or Sp6 or T3.A 5-primer with[阅读全文]

摘要:1 ml linearized DNA template pBS-SK- ERD (X-E) (0.1-0.5 mg)7.8 ml DDW5 ml 5X transcription buffer ( stratagene )1 ml 750 mM DTT1 ml Rnasin (RNase inhibitor; 15U/ml)1 ml 10 mM ATP (pH 7)1 ml 10 mM CTP [阅读全文]

摘要:Steve HahnLast Modified Fri, Apr 25, 2003Wear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol before use to eliminate [阅读全文]

摘要:N.B: Gloves should be worn at all times during preparation of in vitro RNA. All solutions should be RNase-free i.e. made with DEPC water if home-made or bought specifically to use for RNA work. You wi[阅读全文]

摘要: OverviewWith this protocol, transcripts that were initiated from specific genes by RNA polymerases prior to permeabilization can be measured. Instead of a nuclear extract, permeabilized cells are use[阅读全文]

摘要:In vitro transcription with yeast nuclear extract Steve HahnWear gloves throughout, use RNAse free solutions (either autoclaved or sterile filtered) and clean bench and pipetmen with 95% ethanol befor[阅读全文]

摘要:For more information please visit our homepage www.intelligent-siRNA.com Intelligent siRNA [阅读全文]

摘要: Analysis of the interaction of proteins with either DNA or RNA sequences by in vivo footprinting involves two steps: (i) the in situ modification of nucleic acids by the footprinting reagent and (ii[阅读全文]

摘要:sterilisation of seeds: rinse with 70% EtOH for 30 sec put in 1% bleach (sodium hypochlorite, supplemented by a few drops of Tween-20) for 5 min wash 2-4x with sterile H2O for 1 min plating and growth[阅读全文]

摘要:This protocol is based on Shevchenko A,Wilm M,Vorm O,& Mann M.Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels.Anal Chem 1996,68:850-8.I have used it with success on b[阅读全文]

摘要:A modification of the basic immunofluorescence staining and flow cytometric analysis protocol can be used for the simultaneous analysis of surface molecules and intracellular cytokines at single-cell [阅读全文]