摘要:1. embryos are dissected from timed-pregnant mice from 10.5-11.5 d.p.c. 2. limb buds are microdissected and placed in holding medium (L15 medium supplemented with 1 x MITO+ serum extender) (all media [阅读全文]

摘要:1. embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. 2. metanephroi and associated ureteric buds are microdissected and placed in holding medium (L15 medium supplemented w[阅读全文]