摘要:Materials: Taq (or other) PCR enzyme (5 Units/ul) 　　PCR enzyme reaction buffer 　　Distilled water (nuclease free) 　　DNA to be used as template, such as GAPDH template from Clontech 　　DNA to be used as [阅读全文]

摘要:The polymerase chain reaction (PCR) is invented by K. Mullis in 1985, this technique is used to amplify a sequence of DNA in vitro by simulating the replication procedure of natural DNA in vivo. PCR e[阅读全文]