摘要:1. Pick a single colony from a streak plate and inoculate 25 ml of LB with appropriate antibiotic. Incubate overnight.2. Get an ice bucket ready and put buffer P3 (stored in 4℃) in it. Pour the cultur[阅读全文]

摘要: ♦利用真空方式超快速进行质粒纯化，碱裂解步骤后，利用QIAfilter无须离心即可澄清裂解液，然后离心柱安置于真空底座（例如：QIAvac 24 Plus 见图1）上进行DNA的洗涤和洗脱。Midi and Maxi kit 20 分钟内可纯化24 [阅读全文]