摘要:Riley-Day氏综合征又称家族性植物神经失调症，系一种先天性感觉异常综合征。 【病因和机理】 未明 。属常染色体隐体遗传。可能为神经系统、特别是植物神经系统先天性功能异常。 【临床表现】 特征为丰[阅读全文]

摘要:Ribonuclease Protection Assaycontributed by James McCaughern-Carucci, Yale UniversityMost RNase Protection protocols require an overnight hyb with numerous subsequent clean-up steps. This method requi[阅读全文]

摘要:1. Add appropriate volumes of RNA (<12 ul) plus probe (< 3 ul). Try 5 and 10 ug RNA plus 600 or 800 pg dig labelled probe.2. Include 2-3 yeast RNA samples/probe used. 2ul of 5mg/ml stock.3. Add 20 ul [阅读全文]

摘要:FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at[阅读全文]

摘要:1、标记物在RIA中核素标记抗原，在IRMA中核素标记抗体。抗原有不同种类，根据其化学结构，标记时需用不同的核素和不同的方法。抗体为蛋白质，有利于碘化标记，不同抗体标记方法基本相同。标记抗体的比活度高，提高了分析的灵敏[阅读全文]

摘要:1ml RIPA buffer contains 35μl proteinase inhibitor(sigma cat no P8340) and 10μl phosphatase inhibitorRIPA buffer 最终浓度1M Tris-HCl pH7.5) 5 ml 50mMNaCl 0.87g 0.15 MNa-deoxycholale 0.1g （1%）0.5[阅读全文]