摘要:For quantitating RNA levels from either polyA+ selected RNA or crude RNA preps.Solutions10X Hybridization Buffer0.4 M PIPES 12.1 g PIPES (Sigma #P6757)4.0 M NaCl 23.4 g NaCl10 mM EDTA 2.0 ml 500 mM ED[阅读全文]

摘要:The ribonuclease protection assay (RPA) is a highly sensitive and specific method for the detection of mRNA species. The assay was made possible by the discovery and characterization of DNA-dependant [阅读全文]

摘要: RNase Protection Assay (RPA) BD Biosciences公司， PDF文件http://www.bdbiosciences.ca/canada/downloads/protocols/RPA.pdf[阅读全文]

摘要: RNA酶保护试验(RNase Protection Assay,RPA)是通过液相杂交的方式,用反义RNA探针与样品杂交，以检测RNA表达的技术。与Northern杂交和RT-PCR比较，RPA有以下几个优点：1． 检测灵敏度比Northern杂交高。由于Northern杂交步[阅读全文]

摘要:在一个典型的真核细胞中，约有10-5mg的RNA，其中rRNA占80－85％，小RNA（tRNA，small nuclear RNAs）占15－20％，mRNA约占1-5％。RNA不稳定，容易为RNase降解。[阅读全文]