摘要:Rapid elution of DNA from agarose gels James Movius, Hahn LabLast modified Sun, Nov 1, 1998A method of quickly purifying agarose gel DNA fragments for use in subsequent reactions such as further restr[阅读全文]

摘要:Inoculate 1 ml of L-broth or other rich media with cells of the strain from which genomic DNA is to be isolated. Grow this culture at 37℃ overnight on a roller.Transfer this overnight culture to a 1.7[阅读全文]

摘要:SOLUTIONS: Green and blue solutions from Clone Checker kit (cat.no.11666-013).PROCEDURE: Place 3-6µl of an overnight culture in an Eppendorf tube (or spread part of a bacterial colony on the bot[阅读全文]

摘要:A. Process gel1. Electrophorese sample in agarose gel, 0.5 to 1 cm thick, 0.5-1.2%.2. Expose gel to UV light for 1 min on 265 nM light box (or depurinate in 2 vol of 0.25M HCl for 2X15', and rinse in [阅读全文]

摘要:Horvath and Riezman,Yeast,1994; Gottschling LabSample Buffer: 10 ml 0.06M Tris-HCl,pH 6.8 0.6 ml 1M Tris 6.8 10% (v/v)glycerol 2 ml 50% glycerol 2% (w/v)SDS 2 ml 10% SDS 5% (v/v)2-mercaptoethanol 0.5 [阅读全文]

摘要:Procedure Preheat Extraction Buffer at 60°C. Weigh 100 mg of fresh leaf tissue and grind it to powder in Liquid Nitrogen in a chilled mortar and pestle. Add 0.5 ml of TE buffer to the tube. Centri[阅读全文]