摘要:It is best to do a test ligation without insert, if the background is low, it would not be necessary to screen colonies for insert.1.colony PCR 1) Pick a colony from plate and suspend the colony in 10[阅读全文]

摘要:MediaNeed LB plates (9 cm or 14 cm)to plate the library and do secondary screens.I typically screen about 500,000-1 million plaques for a primary cDNA screen.This fits on 11-22 14 cm plates.I do the s[阅读全文]

摘要:Plate a bacteriophage library at about 30,000-50,000 pfus per 150mm Petri dish by adding an appropriate amount of bacteriophage to 500µl MgSO4-stored E.coli.Dilute bacteria,if needed(ex.VCS237 t[阅读全文]

摘要:Digest DNA in 96-well plate To each well add:4μl 10Xbuffer4μl Enzyme0.4μl Spermidine(0.4M)31.6μl H2 O37℃ 19h, then add 4μl loading dye to each well. Load into 400ml 1% agarose gel immed[阅读全文]