摘要:1.Prepare 1 ml of packed beads by washing them 4 times in 10 ml of d-H2O in a 15 ml tube.The beads are Act.Ultrogel 22 AcA from IBF,and stored in the coldroom.The protein binding site is via glutarald[阅读全文]

摘要:1. Conjugation of carrier (BSA) to peptide(1) Dissolve pure BSA 20mg/ml in 10mM phosphate, pH 7.0.(2) Dissolve MBS-SMCC [m-maleimidobenzoyl N-hydroxysuccinimide ester succinimidyl 4- (N-maleimidomethy[阅读全文]