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摘要:Costa_Cell_Culture_for_Ranavirus.pdf[阅读全文]
摘要:yangbinxia:有没有人用脂质体转染过COS-7细胞呀?我看invergen lipofectamin 2000说明书上说这个细胞系转染效率99%,但我用GFP转染后40小时荧光显微镜下观察转染效率没有那么高仅有30-40%吧。可能我的转染条件不太好,我[阅读全文]
摘要:问:cos-7的转染1、细胞的培养:我们在培养细胞时发现该细胞长的很快,不到2天的时间就长满了;消化的时候老是成块的掉,如果消化时间加长,细胞也能成单个的,但是细胞的生长力又减弱了,请问要怎样来克服这一问题呢?2、转染[阅读全文]
摘要:COS7细胞(猴细胞的一种细胞系)为重组病毒的宿主细胞,是表达SV40大T抗原的非洲绿猴肾细胞株,能支持部分突变型SV40和含SV40 ori的质粒载体的复制,可高水平表达外源基因,常作为瞬时表达宿主。故本实验建立COS7细胞的冻存[阅读全文]
摘要:Contributor: Suprya JayadevDate: Mar. 11, 1991Packing column:1) To 20 g of 100-200 mesh Bio-Sil A silica gel add 80 mls of chloroform.2) Place a small portion of glass wool at the base of the column.3[阅读全文]
摘要:Mouse embryos staining, including: in vitro transcription of riboprobe, embryo fixation and permeabilization, hybridization and post-hybridization washes, immunodetection and development of signal. 完[阅读全文]
摘要:Before you start, have ready the following: - Sterilize 2 pairs of curved forceps, 1 pair of iris scissors and 2 pairs of fine watchmaker forceps. - 6ml syringe filled with DMEM with HEPES, with an 18[阅读全文]