稀释限制性内切酶时,建议使用稀释缓冲液(A,B,C)。建议临用前稀释且稀释终浓度不要小于1,000 units/ml。目录及说明书中标注了每一种内切酶相应的稀释兼容性。
注:以下稀释液不适用于耐热DNA聚合酶。欲了解稀释耐热DNA聚合酶的相关情况,请参见相应章节。
稀释缓冲液成份:
稀释液A: 50 mM KCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 200 µg/ml BSA和50% 甘油 (pH 7.4 @ 25℃)。
稀释液B: 300 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 500 µg/ml BSA和50%甘油(pH 7.4 @ 25℃)。
稀释液C: 250 mM NaCl, 10 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, 0.15% Triton X-100, 200 µg/ml BSA和50%甘油(pH 7.4 @ 25℃)。
* 表示稀释时需要添加MgCl2 至终浓度10 mM
可用稀释缓冲液A进行稀释的酶: Aat II Acc I Acc65 I Aci I Afe I Afl II Ahd I Alw I AlwN I Apa I ApaL I Apo I Asc I Ava I Ava II Bae I BamH I Ban I Ban II Bbv I BbvC I Bcc I BceA I Bcg I BciV I Bcl I BfrB I BfuA I BfuC I Bgl II Blp I Bme1580 I Bmt I Bpu10 I BpuE I BsaA I BsaB I BsaH I BsaJ I
BsaW I BseR I BsiE I BsiW I Bsl I Bsm I BsmF I BsoB I Bsp1286 I BspCN I BspD I BspE I BspH I BsrB I BsrD I BsrG I BssH II BssK I BstAP I BstB I BstE II BstF5 I BstN I BstU I BstX I BstY I Bsu36 I Btg I BtgZ I Bts I Cla I Dde I Dra I Drd I Eae I Ear I Eci I EcoN I Eco0109 I EcoR V
Fat I Fau I Fnu4H I Fok I Hae II Hae III Hga I Hha I Hinf I HinP1 I Hpa I Hpa II Hpy99 I Hpy188 I Hpy188 III HpyCH4 III HpyCH4 IV Kpn I Mbo I Mbo II McrBc Mfe I Mlu I Mly I Mse I Msl I Msp I Nae I N.Alw I Nar I N.BbvC IA N.BbvC IB N.BstNB I Nci I Nco I Nde I NgoM IV Nru I Nsi I
Nsp I Pac I PaeR7 I Pci I PflF I PflM I Pho I Ple I Pme I Pml I PshA I Psi I PspG I PspOM I Pvu II Rsa I Sac I Sac II Sal I Sau3A I Sau96 I Sbf I Sca I ScrF I SexA I SgrA I Sma I Sml I SnaB I Spe I Stu I Sty I Tsp45 I Tsp509 I* Xba I Xcm I Xho I Xma I Xmn I Zra I
可用稀释缓冲液B进行稀释的酶: Acl I Afl III* Ale I Alu I Acu I Apek I Ase I AsiS I Avr II Bbs I Bbv I Bfa I BfuA I Bgl I Bmr I Bpm I* Bsa I BsaX I BseY I Bsg I BsiHKA I BsmA I BsmB I BspM I* Bsr I* BssS I BstZ17 I Cac8 I CviA II Dpn I Dpn II Dra III Eag I Fse I Hinc II Hind III Hph I HpyCH4 V I-Ceu I I-Sce I Kas I* Mme I Mnl I Msc I MspA1 I* Mwo I Nla III Nla IV PI-Psp I PI-Sce I PpuM I Pvu I SfaN I Sfc I Sfo I Sph I StyD4 I Swa I Taq I Tli I Tth111 I Tse I TspR I
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