Silica?Clean-up?of?DNA

Materials:

Silica Suspension:

add 2 g of silica to 15 ml of H₂O

wash 3x by centifugation at 2000 x g for 2 min

estimate vol of silica and resuspend in 2 vol H₂O

Silica Wash Solution:

50 mM NaCl, 10 mM Tris 7.5, 2.5 mM EDTA, 50% Ethanol

6 M NaI

note: light sensitive