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macerate tissue in Eppendorf tube without butter at RT add 400 m l extraction buffer vortex for 4 sec leave sample at RT until other samples are ready (> 1 h) spin in microfuge for 1 min transfer 300 m l of supernatant to different Eppendorf tube (prefilled with 300 m l isopropanole) mix and leave at RT for 2 min spin for 5 min vacuum dry pellet and take up in 100 m l TE use 1-2.5 m l for PCR Remarks: DNA is stable for one year at 4℃ Solutions: Extraction buffer: 200 mM Tris-HCl pH 7.5 250 mM NaCl 25 mM EDTA 0.5% SDS |
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