|
i want to know why exactly tris is used as a buffer when we can use any inorganic salt also to reach pH 8 so what is the function of tris? -------------------------------------------------------------------------------- The pH can get there with anything,sweetie,but it will stay there only with Tris or Hepes -------------------------------------------------------------------------------- Tris interacts with the lipopolysaccharides present on the outer membrane which helps to permeabilize the membrane.This effect is enhanced with the addition of EDTA.You can use other buffers,but this is why you find tris in most protocols for DNA as well as protein extraction. -------------------------------------------------------------------------------- |
→如果您认为本词条还有待完善，请 编辑词条
词条内容仅供参考，如果您需要解决具体问题
（尤其在法律、医学等领域），建议您咨询相关领域专业人士。
0
收藏到: 
