|
Does somebody know how to increase DNA concentration from a too weakly concentrated DNA sample obtained with a Qiagen Extraction Kit (the elution buffer was ddH2O) ?? -BATY- -------------------------------------------------------------------------------- You should precipitate your DNA in 0.1V 3M NaOAc pH5.2 + 3V EtOH place -20℃ 30 min spin 15kxg wash pellet in 70% EtOH then resuspend in 10mM tris pH7.5 with a smaller volume than you started. -milanoj- -------------------------------------------------------------------------------- Elute with elution buffer warmed at 50 -Benoit- -------------------------------------------------------------------------------- easy evaperate in 72℃ that is ok -hello- -------------------------------------------------------------------------------- Baty, I didn't think of this before. The protocol specifically calls for elution in EB buffer (10 mM Tris pH 7.5). Check the pH of your ddH2O. Usually it's pretty acidic and this will lead to low DNA concentrations upon elution. -milanoj- -------------------------------------------------------------------------------- |
→如果您认为本词条还有待完善，请 编辑词条
上一篇Role of TRIS in DNA extraction-Molecular Biology 下一篇Phosphatase?treatment?of?DNA?fragmen
词条内容仅供参考，如果您需要解决具体问题
（尤其在法律、医学等领域），建议您咨询相关领域专业人士。
0
收藏到: 
