摘要:Allen试验[阅读全文]

摘要:ALS的某些措施，如气管插管、建立静脉通路、电除颤等应尽可能早进行。若在医院急诊室中或手术室中，病人发生心脏骤停，BLS和ALS应同时进行。这里讨论用药。 （一）碳酸氢钠　它已不再作为心脏骤停时的第一[阅读全文]

摘要:Author: Suzanne GerttulaDate: March 14,1994 1.Digest between 1 and 10 μg Genomic DNA. I digest at 37 C for about six hours over the course of a day. Spin down briefly, heat to 65 ℃ for 10 min and t[阅读全文]

摘要:I'm looking for a methode to demethyle DNA in vivo , during more than 5 days . All I've read about 5-aza-CdT is for maximum 5 days. Why ?Is there another drug and if yes, how does it work ?thanksElea-[阅读全文]

摘要:Alkaline lysis miniprep1. Grow bacteria overnight in 37℃shaking incubator, with lids very loose and taped on. I normally use 5 ml of liquid medium in a 50ml conical bottom tube. Be sure to include the[阅读全文]

摘要:1.Excise band of interest from a TAE gel; estimate volume by weighing the gel slice.2.Dissolve the gel slice in 3 volumes of NaI (from Geneclean kit)at 55E C for 5 min or until the gel dissolves.3.Add[阅读全文]

摘要:• 以96孔板方式，从同一个细胞或组织样本中同时纯化基因组 DNA和总RNA• 处理 96 个样本，只需不到60 分钟• 从同一样本中纯化高品质的基因组DNA和总RNA• 96孔板方式，高效的操作流程• 高度标准[阅读全文]

摘要:SD大鼠200-250克，用3%戊巴比妥钠（35-40mg/kg体重）腹腔麻醉动物后，固定在脑立体定位仪上（参照大鼠全脑立体定位图谱）。以前囟为零点，选出进刀坐标：前后坐标（AP）1 .8mm，中线旁开（L）坐标1. 0mm，腹侧坐标（V）4. 0mm。首先用[阅读全文]

摘要:（一）仪器开/关机程序1、开机程序　　1．1将压缩机电源电缆连接到仪器后面板标有“压缩机”的插口。　　1．2将仪器的电源电缆与仪器上标有MAIN的插口和电网电源相连，确保电网电压足够。　　1．3将仪器主机开关放在Ⅰ的位[阅读全文]

摘要:一、预冻样品盘或西林瓶盛装的液态样品需放在超低温冰箱中预冻。预冻温度取决于所做样品的冰点，Tpre-freezing Tfreezing point -10℃。如果冰点未知，应在-40℃下冷冻2小时以上。最好过夜。有两种冷冻方式：1.静态冷冻：将样品[阅读全文]

摘要:1、【仪器名称】：ALC-EECG多导同步心外膜电图系统。2、【仪器型号】：ALC-EECG16-32。3、【生产厂家】：上海奥尔科特生物科技有限公司。4、【检测适用范围】：应用于心肌缺血、缺氧及药物防治等研究实验。5、【仪器使用优点、缺点】：特别针对[阅读全文]

摘要:1.Cell Culture Scale-up SystemsMost tissue culture is performed on a small scale where relatively small numbers of cells are required for experiments. At this scale cells are usually grown in T flasks[阅读全文]

摘要:This method works well for 17.5 day mouse embryos. All stains should be made up fresh. Fix embryos in 90% ethanol for at least 1 week, longer if possible. Prior to staining remove skin, and viscera, p[阅读全文]