摘要:Chromatin Immunoprecipitation Assay and PCR(galactose inducible genes)adapted by Wendy Reeves (Hahn Lab)from Marnie Gelbart (Tsukiyama lab)Chromatin PreparationTwo days before prep,inoculate 5ml media[阅读全文]

摘要:1.Crosslink protein DNA complexes in vivoGrow 50ml yeast culture to OD600 0.5-1.0.In fume hood,add 1.4ml 37% fomaldehyde solution to a 50ml conical tube.Fill tube with culture to just below the 50ml l[阅读全文]

摘要:1.Crosslink protein DNA complexes in vivoGrow cells in suspension and collect 5 x108 cells by low speed centrifugation.Resuspend cells in 50ml media.In fume hood,add 1.4ml 37% fomaldehyde solution to [阅读全文]