摘要:What it Introduction　　“Flow” + “Cell” + “Measurement” = FCM (FACS®)　　does:　　Measures cell functionalities and quantities (Fluorescence)　　Measures cell sizes (sc[阅读全文]

摘要:流式细胞仪是进行流式细胞分析的仪器，集电子、计算机、激光、流体理论于一体。流式细胞术（Flow CytoMeter, FCM）是一种在功能水平上对单细胞或其他生物粒子进行定量分析和分选的检测手段，它可高速分析上万个细胞，并能同时[阅读全文]

摘要:Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells by adding 4 ml of -20°C absolute ethanol.[阅读全文]

摘要:Internalization of receptor is measured as the reduction of receptors on the plasma membrane surface. Surface receptors are labeled with fluoresent antibodies which is in turn measured using a flow cy[阅读全文]