摘要:Preparation of Cells1.Prepare or collect between 2x107 cells for each mRNA prep (will yield about 10-20µg of mRNA). If PBMCs from a whole blood sample are to be used, the sample should be prepar[阅读全文]

摘要:REAGENTSGuanadinium Thiocyanate1.0 M Sodium Citrate pH 7.010% Sarcosyl2-mercaptoethanolwater saturated phenol pH 4.0-7.0Na2EDTA MOPS （free acid）2.0 M Sodium Acetate pH 4.0FormaldehydeFormamide10% SDS4[阅读全文]

摘要:Procedure1. Pour buffy coat (see Hint #1) from the bag into a 125 ml conical polypropylene centrifuge tube and dilute to 90 ml with sterile endotoxin-free PBS. 2. Add 15 ml of sterile Dextran/PBS and [阅读全文]

摘要:AP Buffer:100 mM Tris-HCl, pH 9.5 100 mM NaCl 10 mM MgCl2 PTM:PBS 0.1% Tween-20 2 mM MgCl2Since this is generally done in conjunction with lacZ staining, embryo/tissue is usually fixed as per lacZ sta[阅读全文]