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摘要:Buffer TfbI, pH5.8 500ml1.47g KOAC 4.95g MnCl2 3.73g KCl 0.74g CaCl2 60ml Glycerin To make: mix, pH down to 5.8 with diluted (about 0.2M) aectic acid, sterile filter, keep at 4 ℃.TfbII 500ml10.47g Mop[阅读全文]