摘要:溶液中进行的化学反应，特别是生物体内的化学反应，往往需要在一定的PH值条件下才能正常进行。人的各种体液都有一定的PH值，而且不容易改变，因此能保证人体正常的生理活动。人的体液之所以具有一定的PH值，是[阅读全文]

摘要:Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high [阅读全文]

摘要:Phosphatase treatment of DNA fragments .pdf[阅读全文]

摘要:1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake, don't vortex).3. Take aqueous (upper) layer. (If dirty sample, repeat Ph/Chl step until interface is fairly clean).4. Ad[阅读全文]

摘要:Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature AgaroseReference:Favre, D. 1992. Biotechniques vol. 13 1.Cut out slice containing DNA, smallest size possible. 2.Est[阅读全文]

摘要:Materials:phenol:chloroform (1:1)chloroform1.Add an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. 2.Mix well. Most DNA solutions can be vortexed for 10 sec except for h[阅读全文]

摘要:(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The University of Oklahoma,Norman,Oklahoma 73019 broe@ou.edu)Phenol extraction is a common technique used to purify a DNA sample (1).[阅读全文]

摘要:1.Add an equal volume (equal to sample volume)of P/C to sample.2.Mix (shake,don't vortex).3.Take aqueous (upper)layer.(If dirty sample,repeat Ph/Chl step until interface is fairly clean).4.Add equal v[阅读全文]

摘要:1.Crash Individual sand flies in 0.5 ml lysis buffer (50 mM NaCl ,10 mM EDTA,50 mM Tris-HCl pH 8).2.Incubate The sand fly homogenates with 100 ng/ml Rnase at 37 ℃ for 30 minutes.3.Incubate The cell ly[阅读全文]

摘要:READ THROUGH ALL CAUTIONS BEFORE TRYING THIS EXPERIMENTMaterials•Rat liver (fasted rat)•Liquid nitrogen•p-Amino-salicic acid•Phenol mixture•Homogenizer or blender6•Refrig[阅读全文]

摘要:Recipes for the buffers are at the end of this protocol.1.Label your protein with 32Pi.Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to [阅读全文]

摘要:吞噬作用是生物体最古老的，也是最基本的防卫机制之一。对于其要消灭的对象无特异性，在免疫学中称之为非特异性免疫作用。中性粒细胞和单核细胞的吞噬作用很强，嗜酸性粒细胞虽然游走性很强，但吞噬能力较弱。白细胞可以通[阅读全文]

摘要:1编程1.1打开快速电泳仪背面的系统开关，仪器开始进行自检。1.2仪器自检完毕后，按键，输入方法号按键确认。1.3按键开始对分离方法进行编程，利用键控制光标的移动，键和键控制步骤的切换。1.4按键对显色方法进行编程，具体步[阅读全文]

摘要:酸度计简称pH计，由电极和电计两部分组成。使用中若能够合理维护电极、按要求配制标准缓冲液和正确操作电计，可大大减小pH示值误差，从而提高化学实验、医学检验数据的可靠性。一、正确使用与保养电极目前实验室使用的电极都[阅读全文]

摘要:1、一般情况下，仪器在连续使用时，每天要标定一次；一般在24小时内仪器不需再标定。2、使用前要拉下电极上端的橡皮套使其露出上端小孔。3、标定的缓冲溶液一般第一次用pH=6.86的溶液，第二次用接近被测溶液pH值的缓冲液，如被[阅读全文]

摘要:PHS-3C型PH计由主机、复合电极组成，主机上有四个旋钮，它们分别是：选择、温度、斜率和定位旋钮。安装好仪器、电极，打开仪器后部的电源开关，预热半小时。在测量之前，首先对PH计进行校准，我们采用两点定位校准法，具体的步骤如[阅读全文]

摘要:（一）保养1、 pH玻璃电极的贮存 　　短期：贮存在pH＝4的缓冲溶液中； 　　长期：贮存在pH＝7的缓冲溶液中。2、 pH玻璃电极的清洗 　　玻璃电极球泡受污染可能使电极响应时间加长。可用CCl4或皂液揩去污物，然后浸入蒸馏水一昼夜后继[阅读全文]

摘要:摘要：研究了不同的溶液pH值对胶体金颜色!粒径的影响"在不同pH值下通过柠檬酸三钠法制备胶体金溶液，利用紫外/可见光吸收分光光度计对胶体金进行扫描，得到最大吸收峰波长及峰宽；通过透射电镜观察其颗粒直径及分布，再将[阅读全文]

摘要:Hancock Laboratory Methods,Department of Microbiology and Immunology,University of British Columbia, British Columbia, Canada http://www.cmdr.ubc.ca/bobh/showmethod.php?methodid=59 REAGENTS:CHCl3:MeOH[阅读全文]