摘要:Materials:phenol:chloroform (1:1)chloroformAdd an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. Mix well. Most DNA solutions can be vortexed for 10 sec except for high [阅读全文]

摘要:1. Add an equal volume (equal to sample volume) of P/C to sample.2. Mix (shake, don't vortex).3. Take aqueous (upper) layer. (If dirty sample, repeat Ph/Chl step until interface is fairly clean).4. Ad[阅读全文]

摘要:Phenol-based Method for the Isolation of DNA Fragments from Low-Melting Temperature AgaroseReference:Favre, D. 1992. Biotechniques vol. 13 1.Cut out slice containing DNA, smallest size possible. 2.Est[阅读全文]

摘要:Materials:phenol:chloroform (1:1)chloroform1.Add an equal volume of buffer-saturated phenol:chloroform (1:1) to the DNA solution. 2.Mix well. Most DNA solutions can be vortexed for 10 sec except for h[阅读全文]

摘要:(adapted from Bruce A.Roe,Department of Chemistry and Biochemistry,The University of Oklahoma,Norman,Oklahoma 73019 broe@ou.edu)Phenol extraction is a common technique used to purify a DNA sample (1).[阅读全文]

摘要:1.Add an equal volume (equal to sample volume)of P/C to sample.2.Mix (shake,don't vortex).3.Take aqueous (upper)layer.(If dirty sample,repeat Ph/Chl step until interface is fairly clean).4.Add equal v[阅读全文]

摘要:READ THROUGH ALL CAUTIONS BEFORE TRYING THIS EXPERIMENTMaterials•Rat liver (fasted rat)•Liquid nitrogen•p-Amino-salicic acid•Phenol mixture•Homogenizer or blender6•Refrig[阅读全文]