摘要:The following is an outline of the method that I use to manufacture injection needles for ES cell injection. I use high quality borosilicate glass obtained from World Precision Instruments (see vendor[阅读全文]

摘要:Transfer Pipettes Glass used for making transfer pipettes : GC120-15 (350 pieces) by Clark Electromedical Instruments. OUTSIDE DIAMETER: 1.2mm INSIDE DIAMETER: 0.69mm LENGTH: 150mm Agent in Australia [阅读全文]

摘要:The manipulation of embryonic stem (ES) cells to generate targeted mutations via homologous recombination has proved an invaluable resource for researchers from fields as diverse as embryology, immuno[阅读全文]

摘要:完整的实验方法请点击下面的链接下载：08Embedding, sectioning and counterstaining of LacZ embryos.pdf[阅读全文]

摘要:Cells are routinely passaged two days prior to electroporating. Usually one 10 cm plate at approximately 80% confluency will provide enough cells for 1-2 electroporations. Procedure 1. Change medium o[阅读全文]

摘要:Need 1.5-2 x 107 cells from a 2 day culture. 1. Cells are harvested as normal, washed x 1 in PBS then taken up at conc. of 1.2 x 10 7 cells/ml in cold PBS. 2. Take x2 Biorad curvettes A. 0.9 ml (107 c[阅读全文]