摘要:Michael Koelle's Lab,Department of Molecular Biophysics and BiochemistryYale University http://info.med.yale.edu/mbb/koelle/protocols/protocol_subcloning.html[阅读全文]

摘要:Author: Laura-Lee Boodram Source: Laura-Lee Boodram, Department of Life Sciences, The University of the West Indies Abstract: The protocol is simple and fairly rapid. It does not require the use of or[阅读全文]

摘要:Dr. GR Lazo, United States Department of Agriculture http://wheat.pw.usda.gov/~lazo/methods/lazo/met8.html [阅读全文]

摘要:PrincipleThe nucleic acids contained within the gel slice are electrophoresed out of the gel, funneled down into the v-shaped channel and trapped within a high salt cushion resting at the bottom of th[阅读全文]

摘要:HiDoes enzyme digestion before bisulfite treatment make a big difference - i am having a few problems with incomplete conversion.If so, can anyone reccomend me a good 'general' enzyme to use - I know [阅读全文]

摘要:Amberg Lab ,Upstate Medical University http://www.upstate.edu/biochem/amberg/protocols/transformation.html1.Grow cells to 1X10E8 or OD600 of 1.2-1.3. 2.Spin cells at 5,000rpm for 5 min, and wash pelle[阅读全文]

摘要:Equilibriation of PhenolCaution: Phenol and chlorofom are very toxic. Wear gloves, protective eye wear and lab coat. Use hood. Phenol dissolves and denatures proteins. It dissolves protein that is tig[阅读全文]

摘要:This procedure allows the concentration of DNA samples from dilute solution and the removal of unwanted salts from DNA samples.Materials•3M Sodium Acetate buffer, pH 5.2 (store at 4 ℃)•Cold [阅读全文]

摘要:Cepko/Tabin Lab ,Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html Cepko/Tabin Lab ,Harvard University http://axon.med.harvard.edu/~cepko/protocol/mike/E1.html Solutions 10X [阅读全文]

摘要:Abstract: The protocol is simple and fairly rapid.It does not require the use of organic solvents but rather utilizes salt extraction to precipitate contaminating proteins.High quality DNA is obtained[阅读全文]

摘要:Sugden lab,McArdle Laboratory for Cancer Research,University of Wisconsin-Madison Medical School DNA /EMSA.pdf">http://mcardle.oncology.wisc.edu/sugden/Protocols/PDF files/Association of protein with [阅读全文]

摘要:Hahn Lab，The Fred Hutchinson Cancer Research Center and Howard Hughes Medical Institute A -80 degree C frozen stock is prepared from Gibco-BRL DH10B competent cells.From this frozen stock,a freshly st[阅读全文]

摘要:1.O/N (5ml E.coli in LB-->1ml into 200ml LB/37℃2.Grow 2-3H to A660 ~0.3-0.43.Chill cells 10'/ice Spin down 10',5K,4℃4.Wash 1X 200ml 10% glycerol (sterile)40℃; Spin down 10',5K,4℃5.Wash 1X 40 ml 10% gl[阅读全文]

摘要:Protocol for extracting DNA from ES Cells,starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA.NOTE- THIS TAKES A LOT OF TIME if you do the whole plate this w[阅读全文]

摘要:Place numbered Eppendorf tubes in dry ice.Cut 0.5cm from end of tails and place in tubes.Store at -80℃ until analyzed.Add 0.3ml of STE/PK solution to the Eppendorf tubes.Incubate at 55℃ overnight with[阅读全文]

摘要:问：请问哪位仁兄能告知一下EDTA-胰蛋白酶的配制。急用！多谢！答：1、胰酶是0.25%,这是质量体积比，就是说0.25g胰酶溶于100ml PBS,注意，尽量不要用水来溶，因为要保持渗透压。2、EDTA的工作液溶度是0.02%－0.1%，根据细胞消化的[阅读全文]

摘要:Epigenetics represents the science for the studying heritable changes of DNA, not involving changes in DNA sequence that regulate gene expression. 本篇文章来源于网络，如有异议请联系我们，我们将在3个工作[阅读全文]

摘要:酶是指由活细胞产生，能在体外和体内起同样催化的一类具有特殊空间结构的生物大分子，包括蛋白质和核酸等。本篇文章来源于网络，如有异议请联系我们，我们将在3个工作日内作出处理。[阅读全文]

摘要:讲座的内容是EpiTect和PyroMark系列新品，为大家介绍QIAGEN在表观遗传学方面，为可靠检测和精确DNA甲基化状态定量分析提供的新工具。表观遗传学的基本概况和定义，DNA甲基化状态的基础背景知识，以及研究的进展。EpiTect和[阅读全文]

摘要:Embryonic Stem Cells:The Mouse Source-vehicle for Mammalian Genetics [阅读全文]