摘要:Base: Bacto Heart Infusion Broth 1.2% Bacto Yeast Extract0.5%Bacto Peptone0.5%NaCl0.025%Na-DNA0.002%pH is adjusted to 8 and autoclaved. May be stored at room temperature for 4 weeks ADD (to final conc[阅读全文]

摘要:成分邻硝基酚β-D-半乳糖昔（ONPG）60mg（O-Nitrophenyl-β-D-galactopyranoside）0.01mol/L磷酸钠缓冲液（pH7.5）10mL1%蛋白胨水（PH7.5）30mL制法将ONPG溶于缓冲液内，加入蛋白胨水，以过滤法除菌，分装于10mm×[阅读全文]

摘要:1. embryos are dissected from timed-pregnant mice from 10.5-11.5 d.p.c. 2. limb buds are microdissected and placed in holding medium (L15 medium supplemented with 1 x MITO+ serum extender) (all media [阅读全文]

摘要:1. embryos are dissected from timed-pregnant mice from 11.5 d.p.c. to 13.5 d.p.c. 2. metanephroi and associated ureteric buds are microdissected and placed in holding medium (L15 medium supplemented w[阅读全文]

摘要:MaterialInstrumentsSolutions1 Curve Scissor50 ml tube 70% Etoh1 Straight ScissorEtoh Spray bottle1 Bone Scraper1bottle of PBS2 Forceps (1 medium, 1 fine) 5 10 cm plates Sacrifice AnimalsPlace mice[阅读全文]

摘要:Publisher: CRCNumber Of Pages: 288Publication Date: 2006-11-06Sales Rank: 797631ISBN / ASIN: 0849339197EAN: 9780849339196Binding: HardcoverManufacturer: CRCStudio: CRCAverage Rating: Total Reviews:Boo[阅读全文]