摘要:【临床表现】 以怀疑配偶不贞的嫉妒妄想为核心症状。患者个性固执、多疑，好发于30～40岁，患者以许多似是而非的证据证明其配偶另有新欢，但往往说不出具体的对象，为此反复侦察、盘问、跟踪、拷打、症状可持续[阅读全文]

摘要:I have been using double-stranded 36mers form STS to probe high-density PAC/BAC filters. The single-stranded overhangs (14 bases) are filled in with 32 P labelled ATP and CTP. POsitive clones are then[阅读全文]

摘要:1. Add 400 μl of TE buffer then 400 μl of 1-butanol to the oligonucleotide glass vial.2. Vortex well, then spin down in tabletop centrifuge for 10 minutes at ~2,000. rpm¹s.3. Remove top, bu[阅读全文]

摘要:SolutionsGel StocksDiluent 5X Buffer 25% Acrylamide209 g Urea 209 g Urea 209 g Ureaup to 500 ml Q 250 ml 10X TBE 120.8 g Acrylamideup to 500 ml Q 4.1 g BISup to 500 ml Q2.5 M NH4 OAc19.2 g NH4 OAcup t[阅读全文]

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文]

摘要:This is a question asked quite a bit, most recently (late September, 1995) in the newsgroup. Here are two methods, and who submitted them. UV Shadowing Electrophorese the oligo via PAGE. Place the gel[阅读全文]

摘要:Use of oligonucleotides in various research applications requires certain basic storage and handling techniques in order to ensure trouble-free experiments. Proper storage of your oligonucleotide will[阅读全文]

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文]

摘要:Purpose: Purification of oligonucleotides (which have already been purified by reverse phase) can increase the efficiency of site directed mutagenesis from around 30% to ~75% or greater in some cases [阅读全文]

摘要:This procedure isolates DNA from agarose gels by filtration through a filter-paper column. The column is made in a 500 μl tube from a slurry of filter paper in TE buffer.Materials Whatman 3MM filte[阅读全文]

摘要:GEL PURIFICATION OF OLIGONUCLEOTIDES(Adapted from the method of Mark Fortini)1. Quantitate crude oligo solution via UV spectrophotometry. Assume 1.0 A260 unit is equal to 33μl/ml. Dry down 100μl of ol[阅读全文]

摘要:TRANSFORMATION (ONE-STEP PEG METHOD)1.Dilute 1:100 a fresh O/N culture of bacteria into prewarmed LB broth and incubate cells with shaking (225 rpm)to an OD600 of 0.3-0.4.2.Add an equal volume of ice-[阅读全文]

摘要:主要作用：预测家畜基因组中特定基因的位置，确定潜在候选基因的数量形状位点，产生新的微卫星引物。[阅读全文]

摘要:全自动生化分析仪的保养十分重要。我院于1996年购进OLYMPUS AU600全自动生化分析仪一台，经过几年的使用，现将日常保养心得介绍如下：1、每日保养。(1)检查样品、试剂是否放好或足够。 (2)检查冲洗样品针、试剂针、搅拌棒。 (3)[阅读全文]

摘要:OLYMPUSAU560生化分析仪系90年代早期产品，故障自检功能不很完善，本文所述均未显示故障所在。故障现象一：测试结果偏低。目测机器工作程序正常，经仔细检查发现样品加样针聚脂管与加样臂上下左右往返运动长期摩擦造成气孔[阅读全文]

摘要:大型医院供应室一般具有相当规模的灭菌设备；本人从事供应室设备维修已有一段时间，现将一常见故障的解决方法提供给各位同仁，希望对大家有所帮助！BD实验故障：常见问题为BD试纸中间发浅，BD试纸是检验灭菌温度、湿度、真空[阅读全文]

摘要:一 、使用注意事项 1、本系统由精密部件组成，操作时要小心，避免突然的或剧烈碰撞和震动。 2、所用的超高压水银汞灯应使用奥林巴斯提供的USH10ZD DC灯（USHIO.INC制造）。 3、确保高压汞灯安装牢固，电线连接紧密。 4、在高压汞[阅读全文]

摘要:1、型号为CH30RF200的OLYMPUS生物显微镜有4个接物镜头，分别为EA4、EA10、EA40和EA100，其中EA100为油镜头，并刻有“oil”标记，镜头较长。2、低倍镜和高倍镜的使用方法同其他光学显微镜的一致。3、在使用显微镜油镜[阅读全文]

摘要:仪器名称OLYMPUS荧光显微镜型　　号BX51TF 国别厂家日本 OLYMPUS公司 主要指标 1、目镜放大倍数10X； 2、物镜放大倍数分别为 4，10，20，40，100X（油镜）； 3、制冷CCD摄像头（300万像素）与IBM电脑连机操作； 4、除明场（Bright Fiel[阅读全文]

摘要:生命经纬网友dwp123的问题为：我实验室有广州光学仪器厂生产的倒置显微镜一台和数码相机一台，想给细胞拍照，有人说直接从目镜中拍摄，有资料说需要一转换接口，有没有哪位院友有这方面的经验，可否介绍介绍，能不能提供转[阅读全文]