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搜索“TAG:re”找到相关内容55篇,用时0.018710秒
摘要:一、实时荧光Taqman 探针设计总原则:探针选择要保守,引物选择要保守,因此必须找一段100-200bp相对要保守的片段来设计引物与探针。即real-time PCR的扩增片段是50bp----150bp。当找不到150bp的保守片段时,必须确保探针的[阅读全文]
摘要:RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation currently available. Compared to the two other commonly used techniques for[阅读全文]
摘要:实时荧光定量PCR原理所谓实时荧光定量PCR技术,是指在PCR反应体系中加入荧光基团,利用荧光信号积累实时监测整个PCR进程,最后通过标准曲线对未知模板进行定量分析的方法。1.内标在传统定量中的作用由于传统定量方法都是[阅读全文]
摘要:自90年代Taqman探针诞生以来,虽然荧光探针(引物)不断有新的技术出现,但是作为一种经典的定量PCR技术,Taqman探针技术仍然是许多实验研究人员进行定量检测的首选,这主要是因为相对于SYBR荧光染料,Taqman探针具有序列特[阅读全文]
摘要:Quantitative RT-PCR is an important step for the validation of expression data generated by cDNA microarray screening and other gene discovery techniques. This has been facilitated by the development [阅读全文]
摘要:Real-time Quantity PCR 本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]
摘要:The real-time machine not only monitors DNA synthesis during the PCR, it also determines the melting point of the product at the end of the PCR. The melting temperature of a DNA double helix depends o[阅读全文]
摘要:In this presentation, we will be using Sybr green to monitor DNA synthesis. Sybr green is a dye which binds to double stranded DNA but not to single-stranded DNA and is frequently used to monitor the [阅读全文]
摘要:A Rotor-Gene real-time genetic analyzer. (型号 Rotor-Gene 6000 real-time analyzer)New updated video shows how the innovative rotary format works: reaction formats, optics, heating, cooling etc Real-ti[阅读全文]
摘要:1、PCR和real-time PCR的基础知识2、实验设计3、数据分析4、PCR和real-time PCR在药物研发中的应用详情请见PPT。 Realtime-PCR基础知识介绍本篇文章来源于网络,如有异议请联系我们,我们将在3个工作日内作出处理。[阅读全文]
摘要:通过一些图表及简短的文字说明,介绍了实时PCR检测方法的原理及探针染料两种标记技术。[阅读全文]
摘要:描述:READIT®单核苷酸多态性基因分型系统是以中-高通量形式检测序列变化/基因型的一个准确、多用途的方法。READIT® 检测以酶忠实性,探针杂交严密性和一体化数据计算器的综合优势,为您的数据提供无比信心。论证[阅读全文]
摘要:关于Red重组系统的简介及其载基因敲除中应用点击链接red同源重组的研究进展.pdf[阅读全文]
摘要:Phosphate-buffered saline (PBS) (0.01 M Na2HPO4 , 0.15 M NaCl, pH 7.4):sodium phosphate dibasic (Na2HPO4) 0.87 gsodium phosphate monobasic (NaH2PO4 x H2O) 0.54 gsodium chloride 8.5 gHPLC water 1 liter[阅读全文]