摘要:1. For double-stranded DNA templates:a. Denature template: 10ml DNA (5-10g or alkaline lysis mini prepDNA)8ml ddH2O　　2ml 2N NaOH　　-incubate 30' at 37℃.b. Dry-ice precipitate: +10ml 4M NH4OAc100ml EtOH[阅读全文]

摘要:一、质粒的提取及鉴定（一）质粒的提取及鉴定1、收获细菌（1）将2ml含相应抗生素的LB液体培养基加入到通气良好的15ml的试管中，接入一单菌落，于37℃剧烈振荡培养过夜。（2）将1.5ml培养物倒入1.5ml离心管中，用台式离心机于4℃以1[阅读全文]

摘要:Below we present the protocols we have used to isolate DNA from various tissues using Silica and Guanidinium Thiocyanate.These protocols are adapted from Boom et al. (1990),Höss & Pääbo[阅读全文]

摘要:Phenol (removes protein) 1.add equal volume of Phenol (= tris-saturated Phenol-Chloroform-Isoamyethanol) 2.vortex 3.spin 2 minutes at 12000 rpm 4℃4.transfer supernatant to a fresh tube (avoid aspirati[阅读全文]

摘要:I am seeking procedures and/or advice on how to isolate human genomic DNA from platoregistry sera. The sera is lymphocytes that have been frozen for 35-40 years but are not dessicated.Will the isolate[阅读全文]

摘要:Abstract: Single strand conformational polymorphism (SSCP)is the most widely used PCR-based methods for point mutation detection. The abnormal band found by SSCP analysis is normally verified using se[阅读全文]

摘要:Tissue collection, storage, microdissection, sectionin g: See separate protocol.Tissue handling : Note that all fresh tissue shoμld be handled as BioSafety Level 2 materials (wear gloves, lab coat,[阅读全文]

摘要:- make a 5µm section to do an evaluation of the % tumour cells- make 50X50µm sections for the DNA isolation- put sections in a falcon tube containing 10 ml of 1XSE- add 100µg/ml prot[阅读全文]

摘要:Does anyone have expereince of extracting DNA from urine? I am having problems with this. I have previously extracted the DNA but then it degraded very quickly and since then I havent even been able t[阅读全文]

摘要:Caltech Genome Research Laboratory,California Institute of Technology http://www.tree.caltech.edu/protocols/agfp.html Restriction digests consist of:15.75 ml ddH2O 1 µl 10 X buffer B (Boehringer[阅读全文]

摘要:I usually use Linear polyacrylamide as a carrier for DNA precipitation by EtOH. I have read about using glycogen as a carrier, I would like to know if anyone knows a method avoiding not to use acrylam[阅读全文]

摘要:I am trying to extract DNA from mammalian bloodbut while doing the phenol extractionlot of Gelatinous material in aqeous phase creating problem in its separation.Can somebody tell me how to minimise i[阅读全文]

摘要:限制性核酸内切酶 ：识别DNA特定序列，切断DNA链 ；DNA聚合酶Ⅰ： 或Klenow1、缺口平移制作标记DNA探针； 2、合成cDNA的第二链；3、填补双链DNA3’凹端；4、DNA序列分析耐热DNA聚合酶（Taq DNA聚合酶等） 聚合酶链反应（PCR）；D[阅读全文]

摘要:限制性内切酶能特异地结合于一段被称为限制性酶识别序列的DNA序列之内或其附近的特异位点上，并切割双链DNA。它可分为三类:Ⅰ类和Ⅲ类酶在同一蛋白质分子中兼有切割和修饰(甲基化)作用且依赖于ATP的存在。Ⅰ类酶结合于识别[阅读全文]

摘要:TaKaRa的内切酶和NEB的内切酶哪个更好一些？ 参考见解： TaKaRa的内切酶、NEB的内切酶两个公司的酶的品质都非常好。NEB公司的酶的活性很高，切出两条小带可能是因为出现星活性，可以试试把酶量减半。NEB的酶很多都是克隆的，[阅读全文]

摘要:Table of Contents10X TBE 40% Acrylamide Stock Alkaline lysis solution 10X TBE:216 g Tris base 110 g boric acid 16.6 g EDTA Add water to 2 liters.40% Acrylamide/Bisacrylamide (40% A&B):38 g Acrylamide [阅读全文]

摘要:Most recent version of protocol: 2/14/96Dr. Paul J. Zambino, Research Plant Molecular Pathologist,U.S.D.A. Forest Service, North Central Forest Experiment Station,Forestry Sciences Laboratory, 5985 Hw[阅读全文]

摘要:DNA Extraction from Archival Formalin-fixed, Paraffin-embed allowfullscreen='true'ded Tissue SectionsAuthor: Shi et al. Source: Contributed by APostodoc Abstract: Describes two methods of extracting D[阅读全文]

摘要:在体外将DNA分子片段与载体DNA片段连接，转入细胞获得大量拷贝的过程中DNA分子克隆(或基因克隆)。其基本步骤包括：制备目的基因→将目的基因与载体用限制性内切酶切割和连接，制成DNA重组→导入宿主细胞→筛[阅读全文]

摘要: I am working with birds of prey....i am looking for a simple method for isolating genomic DNA from avian breast muscle....I have found protocols for mammalian tissue...will these work fine? also i ha[阅读全文]