摘要:Adapted from Frommer et.al.* 1.Dilute DNA (up to 2 mg) into 50 ml with distilled H2O. 2.Add 5.5 ml of 2M NaOH. 3.Incubate at 37℃ for 10 minutes (to create single stranded DNA ). 4.Add 30 ml of 10 mM h[阅读全文:]
摘要:The restriction/modification system in bacteria is a small-scale immune system for protection from infection by foreign DNA.W. Arber and S. Linn (1969)Plating efficiencies of bacteriophage lambda (l p[阅读全文:]
摘要:Pyrosequencing 在肿瘤基因甲基化研究的应用 甲基化研究对于基因的调控和肿瘤的发生有非常密切的关系。在一般正常的细胞中，基因调控区CpG岛处于非甲基化的状态。而当细胞发生癌变后，这些CG区域往往呈现甲基化状态。英国[阅读全文:]
摘要:Can anyone recommend a good kit for genome walking? I would like to find out the promoter sequence of a known gene. Is genome walking the way to do it?thanks a million for any suggestions!-kiwi-Kiwi-I[阅读全文:]
摘要:用于Genome Walking Kit的模板总DNA 一般要满足以下几点：1、DNA 的完整性，电泳为单一条带。2、避免杂质，OD260/280=1.8~2.0。3、模板DNA 不要被污染。4、准确定量，不要少于3μg。[阅读全文:]
摘要:Publisher: Bios Scientific Publishers LtdNumber Of Pages: 608Publication Date: 2002-06-15Sales Rank: 4476658ISBN / ASIN: 1859962289EAN: 9781859962282Binding: HardcoverManufacturer: Bios Scientific Pub[阅读全文:]
摘要:CLONE ISOLATION PREP - PROTOCOLBefore you begin isolating DNA from the cultures:--GENTLENESS is key through step 10!--Try to do prep steps 1-8 at the side of the centrifuge--Make Solution III and plac[阅读全文:]
摘要:点击浏览该文件[阅读全文:]
摘要:ProcedureGrind in mortar and pestle or Waring blender with 5-7 volumes buffer A per g tissue. Use MCE at 350 l/L, and if necessary, with 5 ml 1 M DIECA/L. Squeeze through cheesecloth, two layers of Mi[阅读全文:]
摘要:Protocol for extracting DNA from ES Cells, starting from the 96-well plate but processing in an eppendorf tube to recover more of the DNA . NOTE- THIS TAKES A LOT OF TIME if you do the whole plate thi[阅读全文:]